Plants play a crucial role in the development of soil fungal communities in the remediated substrate after EDTA washing of metal-contaminated soils

In this study, we investigated the importance of plant cover for secondary succession and soil fungal community development in remediated substrates after EDTA washing metal-contaminated soils. The abundance total community, determined by ITS marker genes (Internal Transcribed Spacer region), root colonisation arbuscular mycorrhizal (AM) fungi were monitored two types material ( calcareous acidic ) sown with perennial ryegrass Lolium perenne L.) without bulk ). Four months start experiment, clearly showed that presence plants was main factor affecting which increased rhizosphere most treatments, while it remained at a low level (without plants). Interestingly, addition environmental inoculum, i.e., from semi-natural meadow, did not have positive effect on community. While detected soils end first growing season, structures scarce roots all treatments throughout season. However, second more than year AM virtually frequency colonised length ranging 30% to >75% some latter also soil. This study demonstrates communities soil, has important implications revitalisation regenerative agriculture.